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The reverse primer is designed so that the 5´finishes of The 2 primers anneal backto-back. B) Deletions are engineered by developing normal, non-mutagenic forward and reverse primers that flank the area for being deleted. C) Insertions lower than or equivalent to 6 nucleotides are included in to the 5´ conclusion https://categoryhealth86531.wikiusnews.com/322504/the_smart_trick_of_site_directed_mutagenesis_that_no_one_is_discussing


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