The introduction (or ablation) of a restriction site through mutagenesis vastly facilitates the subsequent strategy of screening for succesfully mutated clones. Forward and reverse primers are created to be complementary, but Each individual primer may well increase further than the complementary region given that an overlap using a least six https://site-directed-mutagenesis31974.blogdigy.com/not-known-facts-about-mutagenesis-35846154